If the problem is originated from both woman and man in the couple, and both of
them are infertile and cant have baby, embryo donation is used for getting pregnancy.
The general success rate is more than 80% in IVF cycles with embryo donation.
So every woman and man can get their baby in every age and every condition.
To which couples embryo donation is performed?
-to women who have uterus but undergoing pre-menopause
-to women who undergoing menopause
-to women that their ovaries are taken out for any reason
-to women whose ovaries lost their activity because taking radiotherapy or chemotherapy
-to women whose eggs are low in quantity and quality
-to women who have recurrent miscarriages
-to women who have genetic disorders that can be inherited to their babies
and when the partner (father candidate)
-have no any sperm in their ejaculate
-have sperm cells but their cells have no fertilization capacity
-have genetic disorders that can be inherited to their babies
-that there is no any sperm in their TESA/TESE tissues
How to use sperm and egg donors for donation (embryo donation) ?
After deciding that embryo donation is necessary for the patient couple, the egg donor is selected from the donor list of the center that consists of the women who applied for sharing their eggs. While selecting the suitable donor it is necessary to be careful about physical and ethnical properties of the couple. And sperm donor is selected from accredited sperm banks according to family candidates properties and their special demands is well.
After selecting the donors, menstrual periods of both egg donor and mother candidate is programmed to be gone together and sperms are shipped from the banks to fertility centers. The eggs are induced in egg donor, while uterus is prepared in mother candidate and after collecting eggs from donor on egg collecting day they fertilized with sperms of the selected sperm donor, then the embryos are transferred on day 3 or 5 of their growing into the uterus of mother candidate. And couple starts for waiting pregnancy test on 12. day after transfer.
The Requirements for Egg Donors:
1- the age of egg donor must be 20-35
2- donor must be physically healthy
3- the body mass index must be 19-29
4- donor must be psychologically healthy
5- donor shouldn't have drug dependent
6-donor shouldn't have any inherited genetic disorder
7-donors ovaries both should be healthy
8-should have karyotype analysis result 46, XX
9-required genetic and laboratory test are performed (Anti-Hiv 1, 2, HbsAg, Anti HBc, Anti-HCV, Syphilis, and sometimes if required HTLV-1 RhD, CMV, T.cruzi, cystic fibrosis, thalasemia, ...)
The Requirements of Sperm Donors:
1- the age of sperm donor must be 20-45
2-the karyotype analysis result must be 46XY
3-physical and ethnical properties must be known
4-required genetic and laboratory test are performed (Blood Type, Thalassamia, Cystic fibrosis, SickleCell, Anti HIV1 and Anti HIV2, HBSAg, Anti HBc, Anti HCV, VDRL-RPR, HTLV-1/II Antibody, Chlamidia, Gonore..)
How both egg and sperm (Embryo) Donation is performed?
Embryo donation consists of three stages;
1-Deciding that we have to use embryo donation and making PRE-PREPARATION:
making required tests and getting reports for mother candidate (FSH, LH, Estradiol, Prolaktin on day 2 or 3 of menstrual bleeding, AMH on any day) and be sure that egg donation is needed. Beside these, making required tests for father candidate and also decide that sperm donation is required (spermiogram , TESE/TESA Reports, FSH, LH, Testesteron). After this, starting to use some medicine to woman partner to refresh and strengthen the uterus. Donor is selected and bleeding time programing is performed for donor and mother candidate to start bleeding on same day. If the mother candidate is in menopause, bleeding is not required for her.And during this time, selected sperms are shipped from the banks (This stage takes 1 week - 1 month and can be performed in patients' country).
on day 2 or 3 of bleeding (menstrual cycle, for both donor and mother candidate) in the month that couple decide to come for the treatment, making required extra tests to both women and start using some more medication like injections for inducing eggs in donor and some medicine for mother candidate for preparing the uterus for embryo. Generally this medication stage takes 10 days. If the mother candidate is in menopause, bleeding is not required for her (this stage takes nearly ten days and can be performed in patients' country). After 12 days (on 14. day of bleeding) couple must be in Cyprus for oocyte pick up (OPU, egg collecting). Then selected sperms are ready to use in the laboratory of the Center.
this starts with oocyte pick up (egg collection) from egg donor and ends with embryo transfer on day 3 or 5 of embryo development to the uterus of mother candidate. Couples must be here on the egg collection day or one day before. The eggs that are collected from donor woman are fertilized with the donor sperms on OPU Day (Day 0). After 3 or 5 days, embryos are transferred to the uterus of mother candidate. After transfer operation, couples can return to their countries whenever they want (this stage takes 3-5 days and patients must be in Cyprus). On 12. day after transfer beta-HCG test in blood must be done for learning pregnancy is negative or positive.